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KMID : 0376219740110010237
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Chonnam Medical Journal
1974 Volume.11 No. 1 p.237 ~ p.245
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Vaginal Fluid NAD Glycohydrolase Activity in the Diagnosis of Cervical Cancer
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Abstract
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Some factors that will influence the activity of NAD glycohydrolase(NADase) in the vaginal fluid obtained by irrigation of the vagina were examined, and the enzyme activity was measured in 10 patients with established carcinoma of the cervix uteri and 29 patients with other non-malignant gynecological disorders.
NADase activity was localized only in the supernatant fluid obtained by centrifugation at 5, 000 x g of both the vaginal fluid and cervical cancer tissue homogenate, with none of the activity found in the precipitate. This finding suggested that cancer cells, when desquamated from the tissue, have liberated its NADase into the supernatant fluid.
NADase activity of human erythrocytes was found to be negligibly low, and human leucocytes were devoid of the enzyme. Accordingly, these blood cells, when mixed with the vaginal fluid, did not affect the vaginal NADase activity
Twenty-four cases out of 29 non-malignant patients did not show any measurable vaginal NADase activity. In sharp contrast, 9 out of 10 malignant cases showed a measurable activity. Thus, the false-positive rate in the non-malignant group was. 14%, and the false-negative rate in the malignant group 10%. Rough correlation existed between vaginal NADase activity and Papanicolaou cytology Class.
It can be stated that the estimation of vaginal NADase activity has some: advantages over the hitherto-reported beta-glucuronidase, 6-phosphogluconate dehydrogenase and ribonuclease in that NADase offers simpler assay method, is immune- to the presence of blood cells, and has lower false-positi a and negative rate in the, discrimination Between non-malignant and malignant groups.
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KEYWORD
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